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KMID : 0352719870110010024
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Journal of Ginseng Research
1987 Volume.11 No. 1 p.24 ~ p.31
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The Detection of Superoxide Dismutase Activity and Isozyme Pattern of Panax ginseng C.A. Meyer Leaves
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Abstract
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We- studied a assay method on the measurement of superoxide dismutase (SOD Superoxide : superoxide oxidoreductase, EC. 1. 15. 1. 1) activity with photoreduced flavin and nitroblue tetrazolium (NBT) as superoxide (OD source and detector, respectively. The AE (1000 ng SOD min.)` of photoreduced flavin-NBT system was 0.08, whereas that of xanthine-xanthine-cytochrome system used broadly in experiments was * 0.014. Therefore, the new method was regarded more simple and utilizable than xanthine-xanthine cytochrome system method. In the present paper, we also carried out to investigate the SOD activity and isozyme pattern for the purpose of study of leaf-burning disease in ginseng (Panax ginseng C.A. Meyer) leaves.
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